The combination of micron and nanotopography by H2SO4/H2O2 treatment and its effects on osteoblast‐specific gene expression of hMSCs
Identifieur interne : 004F91 ( Main/Exploration ); précédent : 004F90; suivant : 004F92The combination of micron and nanotopography by H2SO4/H2O2 treatment and its effects on osteoblast‐specific gene expression of hMSCs
Auteurs : Gustavo Mendonça [Brésil, États-Unis] ; Daniela B. S. Mendonça [Brésil, États-Unis] ; Francisco J. L. Aragão [Brésil] ; Lyndon F. Cooper [États-Unis]Source :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2010-07.
Descripteurs français
- Wicri :
- topic : Biomatériau, Titane.
English descriptors
- KwdEn :
- Acid etching, Adherent, Adherent hmscs, Aluminum oxide particles, Amorphous titanium oxide, Atomic force microscopy, Available implants, Baseline, Baseline levels, Biomaterials, Biomed, Biomed mater, Biomedical, Biomedical materials research part, Bone formation, Carbide papers, Cell behavior, Chapel hill, Chemical treatment, Data points, Dental implants, Descriptive statistics, Different surfaces, Disk surfaces, Expression levels, Gene expression, Grit, Growth media, Gure, Hmscs, Human mesenchymal, Implant, Implant surface, Important role, Machined, Machined surface, Mater, Maxillofac, Mendonca, Mesenchymal, Micron, Mrna, Mrna expression, Mrna levels, Nano, Nano group, Nano surface, Nano surfaces, Nanofeatures, Nanophase ceramics, Nanoscale, Nanoscale features, Nanostructured, Nanostructured surfaces, Nanostructured titanium disks, Nanotopography, Novel nanostructures, Online, Online issue, Oral maxillofac implants, Osseointegration, Osseointegration process, Osteoblast, Osteoblast adhesion, Osteoblast differentiation, Osteoblast function, Osteoblastic differentiation, Osteogenic, Osteogenic media, Osteogenic supplements, Oxidative treatment, Prosthet dent, Pure grade, Relative absence, Roughness parameter, Runx2, Scanning electron micrographs, Several investigations, Smooth surface, Statistical analysis, Subsequent acid, Subsequent acid etching, Surface treatments, Surfaces preparation, Time point, Titanium, Titanium disks, Titanium implants, Titanium surface topography, Titanium surfaces, Topography changes, Universidade catolica, Wiley periodicals.
- Teeft :
- Acid etching, Adherent, Adherent hmscs, Aluminum oxide particles, Amorphous titanium oxide, Atomic force microscopy, Available implants, Baseline, Baseline levels, Biomaterials, Biomed, Biomed mater, Biomedical, Biomedical materials research part, Bone formation, Carbide papers, Cell behavior, Chapel hill, Chemical treatment, Data points, Dental implants, Descriptive statistics, Different surfaces, Disk surfaces, Expression levels, Gene expression, Grit, Growth media, Gure, Hmscs, Human mesenchymal, Implant, Implant surface, Important role, Machined, Machined surface, Mater, Maxillofac, Mendonca, Mesenchymal, Micron, Mrna, Mrna expression, Mrna levels, Nano, Nano group, Nano surface, Nano surfaces, Nanofeatures, Nanophase ceramics, Nanoscale, Nanoscale features, Nanostructured, Nanostructured surfaces, Nanostructured titanium disks, Nanotopography, Novel nanostructures, Online, Online issue, Oral maxillofac implants, Osseointegration, Osseointegration process, Osteoblast, Osteoblast adhesion, Osteoblast differentiation, Osteoblast function, Osteoblastic differentiation, Osteogenic, Osteogenic media, Osteogenic supplements, Oxidative treatment, Prosthet dent, Pure grade, Relative absence, Roughness parameter, Runx2, Scanning electron micrographs, Several investigations, Smooth surface, Statistical analysis, Subsequent acid, Subsequent acid etching, Surface treatments, Surfaces preparation, Time point, Titanium, Titanium disks, Titanium implants, Titanium surface topography, Titanium surfaces, Topography changes, Universidade catolica, Wiley periodicals.
Abstract
H2SO4/H2O2 treatment of titanium implants imparts nanofeatures to the surface and alters the osteoblast response. The aim of this study was to evaluate the effect of H2SO4/H2O2 treatment of commercially pure Titanium (cpTi) surfaces on gene expression of human mesenchymal stem cells (hMSCs) differentiated into osteoblasts. Commercially pure grade IV titanium disks (20.0 mm × 1.0 mm) were polished or polished and subsequently treated by grit blasting or grit‐blasting/acid etching with an H2SO4/H2O2 solution. The surfaces were divided into three groups: smooth (S), grit‐blasted (Gb), and nanostructured: grit‐blasted/acid etched (Nano). Surfaces were examined by scanning electron microscopy and atomic force microscopy. HMSCs were grown on the disks. The data points analyzed were at 3, 7, 14, and 28 days. Real‐time PCR was used to measure the mRNA levels of ALP, BSP, Runx2, OCN, OPN, and OSX. The housekeeping gene GAPDH was used as a control. Descriptive statistics were calculated using Microsoft Excel. T‐test was performed for comparison of mRNA levels when compared with S surfaces (p < 0.05). All osteoblast‐specific genes were regulated in surface‐dependent patterns and most of them were upregulated on the Nano surfaces. Runx2 and OSX mRNAs were more than threefold upregulated at days 14 and 28 on Nano. Higher levels for ALP (38‐fold), BSP (76‐fold), and OCN (3‐fold) were also observed on the Nano surfaces. A grit‐blasted surface imparted with nanofeatures by H2SO4/H2O2 treatment affected adherent cell bone‐specific gene expression. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Url:
DOI: 10.1002/jbm.a.32701
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 005B81
- to stream Istex, to step Curation: 005B81
- to stream Istex, to step Checkpoint: 001A93
- to stream Main, to step Merge: 005032
- to stream Main, to step Curation: 004F91
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The combination of micron and nanotopography by H2SO4/H2O2 treatment and its effects on osteoblast‐specific gene expression of hMSCs</title><author><name sortKey="Mendonca, Gustavo" sort="Mendonca, Gustavo" uniqKey="Mendonca G" first="Gustavo" last="Mendonça">Gustavo Mendonça</name></author><author><name sortKey="Mendonca, Daniela B S" sort="Mendonca, Daniela B S" uniqKey="Mendonca D" first="Daniela B. S." last="Mendonça">Daniela B. S. Mendonça</name></author><author><name sortKey="Aragao, Francisco J L" sort="Aragao, Francisco J L" uniqKey="Aragao F" first="Francisco J. L." last="Aragão">Francisco J. L. Aragão</name></author><author><name sortKey="Cooper, Lyndon F" sort="Cooper, Lyndon F" uniqKey="Cooper L" first="Lyndon F." last="Cooper">Lyndon F. Cooper</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:B8351BCAB43926568291548F0C2E5DD0AF0E5FCC</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1002/jbm.a.32701</idno><idno type="url">https://api.istex.fr/document/B8351BCAB43926568291548F0C2E5DD0AF0E5FCC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">005B81</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">005B81</idno><idno type="wicri:Area/Istex/Curation">005B81</idno><idno type="wicri:Area/Istex/Checkpoint">001A93</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001A93</idno><idno type="wicri:doubleKey">1549-3296:2010:Mendonca G:the:combination:of</idno><idno type="wicri:Area/Main/Merge">005032</idno><idno type="wicri:Area/Main/Curation">004F91</idno><idno type="wicri:Area/Main/Exploration">004F91</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The combination of micron and nanotopography by H<hi rend="subscript">2</hi>SO<hi rend="subscript">4</hi>/H<hi rend="subscript">2</hi>O<hi rend="subscript">2</hi> treatment and its effects on osteoblast‐specific gene expression of hMSCs</title><author><name sortKey="Mendonca, Gustavo" sort="Mendonca, Gustavo" uniqKey="Mendonca G" first="Gustavo" last="Mendonça">Gustavo Mendonça</name><affiliation wicri:level="2"><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Católica de Brasília, Pós‐Graduação em Ciências Genômicas e Biotecnologia, SGAN Quadra 916, Módulo B, Av. W5 Norte 70.790‐160‐Asa Norte, Brasília/DF</wicri:regionArea><placeName><region type="state">District fédéral (Brésil)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 404 Brauer Hall, CB #7450, Chapel Hill</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Católica de Brasília, Curso de Odontologia, QS 07, Lt 01–EPCT, Taguating/DF</wicri:regionArea><placeName><region type="state">District fédéral (Brésil)</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Brésil</country></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Gustavo Mendonça, Universidade Católica de Brasília, Pós‐Graduação em Ciências Genômicas e Biotecnologia, SGAN Quadra 916, Módulo B, Av. W5 Norte 70.790‐160‐Asa Norte, Brasília/DF, BrazilLyndon F. Cooper, Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 404 Brauer Hall, CB #7450, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Mendonca, Daniela B S" sort="Mendonca, Daniela B S" uniqKey="Mendonca D" first="Daniela B. S." last="Mendonça">Daniela B. S. Mendonça</name><affiliation wicri:level="2"><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Católica de Brasília, Pós‐Graduação em Ciências Genômicas e Biotecnologia, SGAN Quadra 916, Módulo B, Av. W5 Norte 70.790‐160‐Asa Norte, Brasília/DF</wicri:regionArea><placeName><region type="state">District fédéral (Brésil)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 404 Brauer Hall, CB #7450, Chapel Hill</wicri:cityArea></affiliation></author><author><name sortKey="Aragao, Francisco J L" sort="Aragao, Francisco J L" uniqKey="Aragao F" first="Francisco J. L." last="Aragão">Francisco J. L. Aragão</name><affiliation wicri:level="2"><country xml:lang="fr">Brésil</country><wicri:regionArea>Universidade Católica de Brasília, Pós‐Graduação em Ciências Genômicas e Biotecnologia, SGAN Quadra 916, Módulo B, Av. W5 Norte 70.790‐160‐Asa Norte, Brasília/DF</wicri:regionArea><placeName><region type="state">District fédéral (Brésil)</region></placeName></affiliation><affiliation wicri:level="2"><country xml:lang="fr">Brésil</country><wicri:regionArea>Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Introdução e Expressão de Genes, PqEB W5 Norte 70770‐900, Brasília/DF</wicri:regionArea><placeName><region type="state">District fédéral (Brésil)</region></placeName></affiliation></author><author><name sortKey="Cooper, Lyndon F" sort="Cooper, Lyndon F" uniqKey="Cooper L" first="Lyndon F." last="Cooper">Lyndon F. Cooper</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 404 Brauer Hall, CB #7450, Chapel Hill</wicri:cityArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Gustavo Mendonça, Universidade Católica de Brasília, Pós‐Graduação em Ciências Genômicas e Biotecnologia, SGAN Quadra 916, Módulo B, Av. W5 Norte 70.790‐160‐Asa Norte, Brasília/DF, BrazilLyndon F. Cooper, Bone Biology and Implant Therapy Laboratory, Department of Prosthodontics, University of North Carolina at Chapel Hill, 404 Brauer Hall, CB #7450, Chapel Hill</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Biomedical Materials Research Part A</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A</title><idno type="ISSN">1549-3296</idno><idno type="eISSN">1552-4965</idno><imprint><biblScope unit="vol">94A</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="169">169</biblScope><biblScope unit="page" to="179">179</biblScope><biblScope unit="page-count">11</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-07">2010-07</date></imprint><idno type="ISSN">1549-3296</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1549-3296</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acid etching</term><term>Adherent</term><term>Adherent hmscs</term><term>Aluminum oxide particles</term><term>Amorphous titanium oxide</term><term>Atomic force microscopy</term><term>Available implants</term><term>Baseline</term><term>Baseline levels</term><term>Biomaterials</term><term>Biomed</term><term>Biomed mater</term><term>Biomedical</term><term>Biomedical materials research part</term><term>Bone formation</term><term>Carbide papers</term><term>Cell behavior</term><term>Chapel hill</term><term>Chemical treatment</term><term>Data points</term><term>Dental implants</term><term>Descriptive statistics</term><term>Different surfaces</term><term>Disk surfaces</term><term>Expression levels</term><term>Gene expression</term><term>Grit</term><term>Growth media</term><term>Gure</term><term>Hmscs</term><term>Human mesenchymal</term><term>Implant</term><term>Implant surface</term><term>Important role</term><term>Machined</term><term>Machined surface</term><term>Mater</term><term>Maxillofac</term><term>Mendonca</term><term>Mesenchymal</term><term>Micron</term><term>Mrna</term><term>Mrna expression</term><term>Mrna levels</term><term>Nano</term><term>Nano group</term><term>Nano surface</term><term>Nano surfaces</term><term>Nanofeatures</term><term>Nanophase ceramics</term><term>Nanoscale</term><term>Nanoscale features</term><term>Nanostructured</term><term>Nanostructured surfaces</term><term>Nanostructured titanium disks</term><term>Nanotopography</term><term>Novel nanostructures</term><term>Online</term><term>Online issue</term><term>Oral maxillofac implants</term><term>Osseointegration</term><term>Osseointegration process</term><term>Osteoblast</term><term>Osteoblast adhesion</term><term>Osteoblast differentiation</term><term>Osteoblast function</term><term>Osteoblastic differentiation</term><term>Osteogenic</term><term>Osteogenic media</term><term>Osteogenic supplements</term><term>Oxidative treatment</term><term>Prosthet dent</term><term>Pure grade</term><term>Relative absence</term><term>Roughness parameter</term><term>Runx2</term><term>Scanning electron micrographs</term><term>Several investigations</term><term>Smooth surface</term><term>Statistical analysis</term><term>Subsequent acid</term><term>Subsequent acid etching</term><term>Surface treatments</term><term>Surfaces preparation</term><term>Time point</term><term>Titanium</term><term>Titanium disks</term><term>Titanium implants</term><term>Titanium surface topography</term><term>Titanium surfaces</term><term>Topography changes</term><term>Universidade catolica</term><term>Wiley periodicals</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acid etching</term><term>Adherent</term><term>Adherent hmscs</term><term>Aluminum oxide particles</term><term>Amorphous titanium oxide</term><term>Atomic force microscopy</term><term>Available implants</term><term>Baseline</term><term>Baseline levels</term><term>Biomaterials</term><term>Biomed</term><term>Biomed mater</term><term>Biomedical</term><term>Biomedical materials research part</term><term>Bone formation</term><term>Carbide papers</term><term>Cell behavior</term><term>Chapel hill</term><term>Chemical treatment</term><term>Data points</term><term>Dental implants</term><term>Descriptive statistics</term><term>Different surfaces</term><term>Disk surfaces</term><term>Expression levels</term><term>Gene expression</term><term>Grit</term><term>Growth media</term><term>Gure</term><term>Hmscs</term><term>Human mesenchymal</term><term>Implant</term><term>Implant surface</term><term>Important role</term><term>Machined</term><term>Machined surface</term><term>Mater</term><term>Maxillofac</term><term>Mendonca</term><term>Mesenchymal</term><term>Micron</term><term>Mrna</term><term>Mrna expression</term><term>Mrna levels</term><term>Nano</term><term>Nano group</term><term>Nano surface</term><term>Nano surfaces</term><term>Nanofeatures</term><term>Nanophase ceramics</term><term>Nanoscale</term><term>Nanoscale features</term><term>Nanostructured</term><term>Nanostructured surfaces</term><term>Nanostructured titanium disks</term><term>Nanotopography</term><term>Novel nanostructures</term><term>Online</term><term>Online issue</term><term>Oral maxillofac implants</term><term>Osseointegration</term><term>Osseointegration process</term><term>Osteoblast</term><term>Osteoblast adhesion</term><term>Osteoblast differentiation</term><term>Osteoblast function</term><term>Osteoblastic differentiation</term><term>Osteogenic</term><term>Osteogenic media</term><term>Osteogenic supplements</term><term>Oxidative treatment</term><term>Prosthet dent</term><term>Pure grade</term><term>Relative absence</term><term>Roughness parameter</term><term>Runx2</term><term>Scanning electron micrographs</term><term>Several investigations</term><term>Smooth surface</term><term>Statistical analysis</term><term>Subsequent acid</term><term>Subsequent acid etching</term><term>Surface treatments</term><term>Surfaces preparation</term><term>Time point</term><term>Titanium</term><term>Titanium disks</term><term>Titanium implants</term><term>Titanium surface topography</term><term>Titanium surfaces</term><term>Topography changes</term><term>Universidade catolica</term><term>Wiley periodicals</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Biomatériau</term><term>Titane</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">H2SO4/H2O2 treatment of titanium implants imparts nanofeatures to the surface and alters the osteoblast response. The aim of this study was to evaluate the effect of H2SO4/H2O2 treatment of commercially pure Titanium (cpTi) surfaces on gene expression of human mesenchymal stem cells (hMSCs) differentiated into osteoblasts. Commercially pure grade IV titanium disks (20.0 mm × 1.0 mm) were polished or polished and subsequently treated by grit blasting or grit‐blasting/acid etching with an H2SO4/H2O2 solution. The surfaces were divided into three groups: smooth (S), grit‐blasted (Gb), and nanostructured: grit‐blasted/acid etched (Nano). Surfaces were examined by scanning electron microscopy and atomic force microscopy. HMSCs were grown on the disks. The data points analyzed were at 3, 7, 14, and 28 days. Real‐time PCR was used to measure the mRNA levels of ALP, BSP, Runx2, OCN, OPN, and OSX. The housekeeping gene GAPDH was used as a control. Descriptive statistics were calculated using Microsoft Excel. T‐test was performed for comparison of mRNA levels when compared with S surfaces (p < 0.05). All osteoblast‐specific genes were regulated in surface‐dependent patterns and most of them were upregulated on the Nano surfaces. Runx2 and OSX mRNAs were more than threefold upregulated at days 14 and 28 on Nano. Higher levels for ALP (38‐fold), BSP (76‐fold), and OCN (3‐fold) were also observed on the Nano surfaces. A grit‐blasted surface imparted with nanofeatures by H2SO4/H2O2 treatment affected adherent cell bone‐specific gene expression. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010</div></front></TEI><affiliations><list><country><li>Brésil</li><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>District fédéral (Brésil)</li></region></list><tree><country name="Brésil"><region name="District fédéral (Brésil)"><name sortKey="Mendonca, Gustavo" sort="Mendonca, Gustavo" uniqKey="Mendonca G" first="Gustavo" last="Mendonça">Gustavo Mendonça</name></region><name sortKey="Aragao, Francisco J L" sort="Aragao, Francisco J L" uniqKey="Aragao F" first="Francisco J. L." last="Aragão">Francisco J. L. Aragão</name><name sortKey="Aragao, Francisco J L" sort="Aragao, Francisco J L" uniqKey="Aragao F" first="Francisco J. L." last="Aragão">Francisco J. L. Aragão</name><name sortKey="Mendonca, Daniela B S" sort="Mendonca, Daniela B S" uniqKey="Mendonca D" first="Daniela B. S." last="Mendonça">Daniela B. S. Mendonça</name><name sortKey="Mendonca, Gustavo" sort="Mendonca, Gustavo" uniqKey="Mendonca G" first="Gustavo" last="Mendonça">Gustavo Mendonça</name><name sortKey="Mendonca, Gustavo" sort="Mendonca, Gustavo" uniqKey="Mendonca G" first="Gustavo" last="Mendonça">Gustavo Mendonça</name></country><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Mendonca, Gustavo" sort="Mendonca, Gustavo" uniqKey="Mendonca G" first="Gustavo" last="Mendonça">Gustavo Mendonça</name></region><name sortKey="Cooper, Lyndon F" sort="Cooper, Lyndon F" uniqKey="Cooper L" first="Lyndon F." last="Cooper">Lyndon F. Cooper</name><name sortKey="Cooper, Lyndon F" sort="Cooper, Lyndon F" uniqKey="Cooper L" first="Lyndon F." last="Cooper">Lyndon F. Cooper</name><name sortKey="Cooper, Lyndon F" sort="Cooper, Lyndon F" uniqKey="Cooper L" first="Lyndon F." last="Cooper">Lyndon F. Cooper</name><name sortKey="Mendonca, Daniela B S" sort="Mendonca, Daniela B S" uniqKey="Mendonca D" first="Daniela B. S." last="Mendonça">Daniela B. S. Mendonça</name><name sortKey="Mendonca, Gustavo" sort="Mendonca, Gustavo" uniqKey="Mendonca G" first="Gustavo" last="Mendonça">Gustavo Mendonça</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004F91 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004F91 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Santé
|area= EdenteV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:B8351BCAB43926568291548F0C2E5DD0AF0E5FCC
|texte= The combination of micron and nanotopography by H2SO4/H2O2 treatment and its effects on osteoblast‐specific gene expression of hMSCs
}}
| This area was generated with Dilib version V0.6.32. |  |